| Title: | T cell line infections with HIV-1 LAI (BRU) |
|---|---|
| Description: | The expression levels of approximately 4600 cellular RNA transcripts were assessed in CD4+ T cell lines at different times after infection with HIV-1BRU using DNA microarrays. This data corresponds to the first block of a 12 block array image (001030_08_1.GEL) in the first data set (2000095918 A) in the first experiment (CEM LAI vs HI-LAI 24hr). There are two data sets, which are part of a dye-swap experiment with replicates, representing the Cy3 (green) absorption intensities for channel 1 (hiv1raw) and the Cy5 (red) absorption intensities for channel 2 (hiv2raw). |
| Authors: | Dr. Angelique van't Wout, Department of Microbiology, University of Washington |
| Maintainer: | Chris Fraley <[email protected]> |
| License: | GPL (>= 2) |
| Version: | 1.52.0 |
| Built: | 2026-10-10 10:59:42 UTC |
| Source: | https://github.com/bioc/HIVcDNAvantWout03 |
The expression levels of approximately
4600 cellular RNA transcripts were assessed in CD4+ T cell lines at different
times after infection with HIV-1BRU using DNA microarrays.
There are two data sets, which are part of a dye-swap experiment with
replicates,
representing the Cy3 (green) absorption intensities for channel 1
(hiv1raw)
and the Cy5 (red) absorption intensities for channel 2
(hiv2raw).
data(hiv1raw)data(hiv1raw)
This data represents a block within a microarray image with 12x32 spots. It is stored as a vector of length 450,000 representing a 450x1000 matrix (ordered by column) of intensities encoded for compact (16-bit TIFF) storage.
The intensities can be obtained from this data by first
subtracting them from 65535, then squaring, then multiplying by
a scale factor 4.71542407E-05.
In other words, a number x in the hiv1 data set corresponds
to intensity .
Dr. Angelique van't Wout, Department of Microbiology, University of Washington
The data corresponds to the first block of a 12 block array image (‘001030\_08\_1.GEL’) in the first data set (‘2000095918 A’) in the first experiment (‘CEM LAI vs HI-LAI 24hr’) of the following data archive: http://expression.microslu.washington.edu/expression/vantwoutjvi2002.html
van't Wout AB, Lehrman GK, Mikheeva SA, O'Keeffe GC, Katze MG, Bumgarner RE, Geiss GK and Mullins JI, Cellular gene expression upon human immunodeficiency virus type 1 infection of CD4(+)-T-cell lines, J Virol. 2003 Jan;77(2):1392-402.a
The expression levels of approximately
4600 cellular RNA transcripts were assessed in CD4+ T cell lines at different
times after infection with HIV-1BRU using DNA microarrays.
There are two data sets, which are part of a dye-swap experiment with
replicates,
representing the Cy3 (green) absorption intensities for channel 1
(hiv1raw)
and the Cy5 (red) absorption intensities for channel 2
(hiv2raw).
data(hiv2raw)data(hiv2raw)
This data represents a block within a microarray image with 12x32 spots. It is stored as a vector of length 450,000 representing a 450x1000 matrix (ordered by column) of intensities encoded for compact (16-bit TIFF) storage.
The intensities can be obtained from this data by first
subtracting them from 65535, then squaring, then multiplying by
a scale factor 4.71542407E-05.
In other words, a number x in the hiv1 data set corresponds
to intensity .
Dr. Angelique van't Wout, Department of Microbiology, University of Washington
The data corresponds to the first block of a 12 block array image (‘001030\_08\_1.GEL’) in the first data set (‘2000095918 A’) in the first experiment (‘CEM LAI vs HI-LAI 24hr’) of the following data archive:\ http://expression.microslu.washington.edu/expression/vantwoutjvi2002.html
van't Wout AB, Lehrman GK, Mikheeva SA, O'Keeffe GC, Katze MG, Bumgarner RE, Geiss GK and Mullins JI, Cellular gene expression upon human immunodeficiency virus type 1 infection of CD4(+)-T-cell lines, J Virol. 2003 Jan;77(2):1392-402.