Package: HSMMSingleCell Type: Package Title: Single-cell RNA-Seq for differentiating human skeletal muscle myoblasts (HSMM) Version: 1.32.0 Date: 2014-03-11 Author: Cole Trapnell Maintainer: Cole Trapnell Description: Skeletal myoblasts undergo a well-characterized sequence of morphological and transcriptional changes during differentiation. In this experiment, primary human skeletal muscle myoblasts (HSMM) were expanded under high mitogen conditions (GM) and then differentiated by switching to low-mitogen media (DM). RNA-Seq libraries were sequenced from each of several hundred cells taken over a time-course of serum-induced differentiation. Between 49 and 77 cells were captured at each of four time points (0, 24, 48, 72 hours) following serum switch using the Fluidigm C1 microfluidic system. RNA from each cell was isolated and used to construct mRNA-Seq libraries, which were then sequenced to a depth of ~4 million reads per library, resulting in a complete gene expression profile for each cell. License: Artistic-2.0 biocViews: ExperimentData, RNASeqData LazyLoad: yes Packaged: 2026-10-10 11:04:58 UTC; root Depends: R (>= 2.10) Repository: Bioconductor 3.23 Date/Publication: 2026-04-28 12:27:00 UTC RemoteUrl: https://github.com/bioc/HSMMSingleCell RemoteRef: RELEASE_3_23 RemoteSha: 99b54ba6856f7ee99438dc79dfe6c8470050e06b NeedsCompilation: no