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  "Package": "IsoformSwitchAnalyzeR",
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  "Title": "Identify, Annotate and Visualize Isoform Switches with\nFunctional Consequences from both short- and long-read RNA-seq\ndata",
  "Version": "2.12.0",
  "Authors@R": "c(person(given=\"Kristoffer\",family=\"Vitting-Seerup\", role=c(\"cre\",\"aut\"),email=\"k.vitting.seerup@gmail.com\",comment = c(ORCID = \"0000-0002-6450-0608\")), person(given=\"Chunxu\",family=\"Han\", role=\"ctb\",email=\"chunxuhan007@gmail.com\"), person(given=\"Jeroen\",family=\"Gilis\", role=\"ctb\", email=\"jeroen.gilis@ugent.be\",comment = c(ORCID = \"0000-0001-8415-0943\")), person(given=\"Elena Iriondo\", family=\"Delgado\", role=\"ctb\",email=\"eleni.iriondo2@gmail.com\"))",
  "Description": "Analysis of alternative splicing and isoform switches with\npredicted functional consequences (e.g. gain/loss of protein\ndomains etc.) from quantification of all types of RNA-seq\n(short/long) by tools such as Kallisto, Salmon, StringTie,\nTallon, IsoQuant etc.",
  "URL": "http://bioconductor.org/packages/IsoformSwitchAnalyzeR/",
  "BugReports": "https://github.com/kvittingseerup/IsoformSwitchAnalyzeR/issues",
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  "biocViews": "GeneExpression, Transcription, AlternativeSplicing,\nDifferentialExpression, DifferentialSplicing, Visualization,\nStatisticalMethod, TranscriptomeVariant, BiomedicalInformatics,\nFunctionalGenomics, SystemsBiology, Transcriptomics, RNASeq,\nAnnotation, FunctionalPrediction, GenePrediction, DataImport,\nMultipleComparison, BatchEffect, ImmunoOncology",
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  "Repository": "https://bioc-release.r-universe.dev",
  "Date/Publication": "2026-04-28 12:45:47 UTC",
  "RemoteUrl": "https://github.com/bioc/IsoformSwitchAnalyzeR",
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  "Author": "Kristoffer Vitting-Seerup [cre, aut] (ORCID:\n<https://orcid.org/0000-0002-6450-0608>),\nChunxu Han [ctb],\nJeroen Gilis [ctb] (ORCID: <https://orcid.org/0000-0001-8415-0943>),\nElena Iriondo Delgado [ctb]",
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  "_exports": [
    "addORFfromGTF",
    "analyzeAlternativeSplicing",
    "analyzeCPAT",
    "analyzeCPC2",
    "analyzeDeepLoc2",
    "analyzeDeepTMHMM",
    "analyzeIntronRetention",
    "analyzeIUPred2A",
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    "analyzeNovelIsoformORF",
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    "exportToPairedGSEA",
    "extractConsequenceEnrichment",
    "extractConsequenceEnrichmentComparison",
    "extractConsequenceGenomeWide",
    "extractConsequenceSummary",
    "extractGeneExpression",
    "extractGenomeWideAnalysis",
    "extractSequence",
    "extractSplicingEnrichment",
    "extractSplicingEnrichmentComparison",
    "extractSplicingGenomeWide",
    "extractSplicingSummary",
    "extractSubCellShifts",
    "extractSwitchOverlap",
    "extractSwitchSummary",
    "extractTopSwitches",
    "importCufflinksFiles",
    "importGTF",
    "importIsoformExpression",
    "importPairedGSEA",
    "importRdata",
    "importSalmonData",
    "isoformSwitchAnalysisCombined",
    "isoformSwitchAnalysisPart1",
    "isoformSwitchAnalysisPart2",
    "isoformSwitchTestDEXSeq",
    "isoformSwitchTestSatuRn",
    "isoformToGeneExp",
    "isoformToIsoformFraction",
    "preFilter",
    "prepareSalmonFileDataFrame",
    "subsetSwitchAnalyzeRlist",
    "switchPlot",
    "switchPlotGeneExp",
    "switchPlotIsoExp",
    "switchPlotIsoUsage",
    "switchPlotTopSwitches",
    "switchPlotTranscript"
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      "title": "Example data for IsoformSwitchAnalyzeR",
      "object": "exampleSwitchList",
      "file": "exampleSwitchList.RData",
      "class": [
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      "fields": [],
      "table": false,
      "tojson": false
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      "name": "exampleSwitchListAnalyzed",
      "title": "Example data for IsoformSwitchAnalyzeR",
      "object": "exampleSwitchListAnalyzed",
      "file": "exampleSwitchListAnalyzed.RData",
      "class": [
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      "table": false,
      "tojson": false
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      "name": "exampleSwitchListIntermediary",
      "title": "Example data for IsoformSwitchAnalyzeR",
      "object": "exampleSwitchListIntermediary",
      "file": "exampleSwitchListIntermediary.RData",
      "class": [
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      "fields": [],
      "table": false,
      "tojson": false
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  "_help": [
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      "page": "addORFfromGTF",
      "title": "Add CDS from a GTF file to a switchAnalyzeRlist.",
      "topics": [
        "addORFfromGTF"
      ]
    },
    {
      "page": "analyzeAlternativSplicing",
      "title": "Analyse alternative splicing (including intron retention(s))",
      "topics": [
        "analyzeAlternativeSplicing",
        "analyzeIntronRetention"
      ]
    },
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      "page": "analyzeCPAT",
      "title": "Import Result of External Sequence Analysis",
      "topics": [
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    {
      "page": "analyzeCPC2",
      "title": "Import Result of External Sequence Analysis",
      "topics": [
        "analyzeCPC2"
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    },
    {
      "page": "analyzeDeepLoc2",
      "title": "Import Result of DeepLoc2 Analysis",
      "topics": [
        "analyzeDeepLoc2"
      ]
    },
    {
      "page": "analyzeDeepTMHMM",
      "title": "Import Result of a DeepTMHMM analysis",
      "topics": [
        "analyzeDeepTMHMM"
      ]
    },
    {
      "page": "analyzeIUPred2A",
      "title": "Import Result of IUPred2A or IUPred3 analysis",
      "topics": [
        "analyzeIUPred2A"
      ]
    },
    {
      "page": "analyzeNetSurfP3",
      "title": "Import Result of NetSurfP3 analysis",
      "topics": [
        "analyzeNetSurfP3"
      ]
    },
    {
      "page": "analyzeNovelIsoformORF",
      "title": "Prediction of Isoform Open Reading Frames.",
      "topics": [
        "analyzeNovelIsoformORF"
      ]
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    {
      "page": "analyzeORF",
      "title": "Prediction of Transcript Open Reading Frame.",
      "topics": [
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    {
      "page": "analyzePFAM",
      "title": "Import Result of PFAM analysis",
      "topics": [
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      "page": "analyzeSignalP",
      "title": "Import Result of SignalP Analysis",
      "topics": [
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    {
      "page": "analyzeSwitchConsequences",
      "title": "Analyze Consequences of Isoform Switches",
      "topics": [
        "analyzeSwitchConsequences"
      ]
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    {
      "page": "switchAnalyzeRlist",
      "title": "Create a switchAnalyzeRlist Object",
      "topics": [
        "createSwitchAnalyzeRlist",
        "switchAnalyzeRlist",
        "switchAnalyzeRlist-class"
      ]
    },
    {
      "page": "testData",
      "title": "Example data for IsoformSwitchAnalyzeR",
      "topics": [
        "exampleSwitchList",
        "exampleSwitchListAnalyzed",
        "exampleSwitchListIntermediary"
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    },
    {
      "page": "exportToPairedGSEA",
      "title": "Export Data for pairedGSEA Analysis",
      "topics": [
        "exportToPairedGSEA"
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    },
    {
      "page": "extractConsequenceEnrichment",
      "title": "Analyze data for enrichment of specific consequences",
      "topics": [
        "extractConsequenceEnrichment"
      ]
    },
    {
      "page": "extractConsequenceEnrichmentComparison",
      "title": "Compare enrichment of specific consequences between comparisons",
      "topics": [
        "extractConsequenceEnrichmentComparison"
      ]
    },
    {
      "page": "extractGenomeWideAnalysis",
      "title": "Genome wide Analysis of Consequences due to isoform switching",
      "topics": [
        "extractConsequenceGenomeWide",
        "extractGenomeWideAnalysis"
      ]
    },
    {
      "page": "extractConsequenceSummary",
      "title": "Analyze Switch Consequences",
      "topics": [
        "extractConsequenceSummary"
      ]
    },
    {
      "page": "extractGeneExpression",
      "title": "Extract raw gene counts or abundances from a switchAnalyzeRlist object.",
      "topics": [
        "extractGeneExpression"
      ]
    },
    {
      "page": "extractSequence",
      "title": "Extract nucloetide (and amino acid) sequence of transcripts.",
      "topics": [
        "extractSequence"
      ]
    },
    {
      "page": "extractSplicingEnrichment",
      "title": "Analyze data for enrichment of specific type of alternative splicing",
      "topics": [
        "extractSplicingEnrichment"
      ]
    },
    {
      "page": "extractSplicingEnrichmentComparison",
      "title": "Compare enrichment of specific type of alternative splicing between comparisons",
      "topics": [
        "extractSplicingEnrichmentComparison"
      ]
    },
    {
      "page": "extractGenomeWideSplicingAnalysis",
      "title": "Genome wide Analysis of alternative splicing",
      "topics": [
        "extractSplicingGenomeWide"
      ]
    },
    {
      "page": "extractSplicingSummary",
      "title": "Extracts alternative splicing summary",
      "topics": [
        "extractSplicingSummary"
      ]
    },
    {
      "page": "extractSubCellShifts",
      "title": "Global overview of sub-cellular location changes",
      "topics": [
        "extractSubCellShifts"
      ]
    },
    {
      "page": "extractSwitchOverlap",
      "title": "Visualize Switch Overlap",
      "topics": [
        "extractSwitchOverlap"
      ]
    },
    {
      "page": "extractSwitchSummary",
      "title": "Summarize Isoform Switches test Result.",
      "topics": [
        "extractSwitchSummary"
      ]
    },
    {
      "page": "extractTopSwitches",
      "title": "Extract Top Isoform Switches.",
      "topics": [
        "extractTopSwitches"
      ]
    },
    {
      "page": "importCufflinksGalaxyData",
      "title": "Import CuffDiff (Cufflinks) Data Into R",
      "topics": [
        "importCufflinksFiles"
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    },
    {
      "page": "importGTF",
      "title": "Import Transcripts from a GTF file into R",
      "topics": [
        "importGTF"
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    },
    {
      "page": "importIsoformExpression",
      "title": "Import expression data from Kallisto, Salmon, RSEM or StringTie into R.",
      "topics": [
        "importIsoformExpression"
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    },
    {
      "page": "importPairedGSEA",
      "title": "Import PairedGSEA Results into IsoformSwitchAnalyzeR",
      "topics": [
        "importPairedGSEA"
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    },
    {
      "page": "importRdata",
      "title": "Create SwitchAnalyzeRlist From Standard R Objects",
      "topics": [
        "importRdata"
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    },
    {
      "page": "importSalmonData",
      "title": "Direct creation of a switchAnalyzeRlist from Salmon quantification",
      "topics": [
        "importSalmonData"
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    {
      "page": "isoformSwitchAnalysisCombined",
      "title": "Isoform Switch Analysis Workflow: Extract, Annotate and Visualize all Significant Isoform Switches",
      "topics": [
        "isoformSwitchAnalysisCombined"
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    {
      "page": "isoformSwitchAnalysisPart1",
      "title": "Isoform Switch Analysis Workflow Part 1: Extract Isoform Switches and Their Bio-sequences",
      "topics": [
        "isoformSwitchAnalysisPart1"
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    {
      "page": "isoformSwitchAnalysisPart2",
      "title": "Isoform Switch Analysis Workflow Part 2: Plot All Isoform Switches and Their Annotation",
      "topics": [
        "isoformSwitchAnalysisPart2"
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    },
    {
      "page": "IsoformSwitchTestDEXSeq",
      "title": "Statistical Test for identifying Isoform Switching via DEXSeq",
      "topics": [
        "isoformSwitchTestDEXSeq"
      ]
    },
    {
      "page": "IsoformSwitchTestSatuRn",
      "title": "Statistical Test for identifying Isoform Switching via satuRn.",
      "topics": [
        "isoformSwitchTestSatuRn"
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    },
    {
      "page": "isoformToGeneExp",
      "title": "Sum transcript/isoform expression to gene get level expression.",
      "topics": [
        "isoformToGeneExp"
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    },
    {
      "page": "isoformToIsoformFraction",
      "title": "Calculate isoform fraction from isoform abundance matrix",
      "topics": [
        "isoformToIsoformFraction"
      ]
    },
    {
      "page": "preFilter",
      "title": "Filtering of a switchAnalyzeRlist",
      "topics": [
        "preFilter"
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    },
    {
      "page": "prepareSalmonFilesDataFrame",
      "title": "Prepare data.frame needed run importSalmonData.",
      "topics": [
        "prepareSalmonFileDataFrame"
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    },
    {
      "page": "subsetSwitchAnalyzeRlist",
      "title": "A function which subset all entries in a switchAnalyzeRlist.",
      "topics": [
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    },
    {
      "page": "switchPlot",
      "title": "Isoform Switch Analysis Plot",
      "topics": [
        "switchPlot"
      ]
    },
    {
      "page": "expressionAnalysisPlots",
      "title": "Plots for Analyzing Expression and Isoform Usage",
      "topics": [
        "switchPlotGeneExp",
        "switchPlotIsoExp",
        "switchPlotIsoUsage"
      ]
    },
    {
      "page": "switchPlotTopSwitches",
      "title": "Creating the Isoform Switch Analysis Plot for the Top Switches",
      "topics": [
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    },
    {
      "page": "switchPlotTranscript",
      "title": "Plot Transcript Structure and Annotation",
      "topics": [
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      "source": "IsoformSwitchAnalyzeR.Rmd",
      "filename": "IsoformSwitchAnalyzeR.html",
      "title": "IsoformSwitchAnalyzeR",
      "engine": "knitr::rmarkdown",
      "headings": [
        "Abstract",
        "Table of Content",
        "Preliminaries",
        "Background and Package Description",
        "Installation",
        "How To Get Help",
        "What To Cite",
        "Quick Start",
        "Overview of Isoform Switch Workflow",
        "Example Workflow",
        "Importing the Data",
        "Part 1",
        "Part 2",
        "Examples Visualizations",
        "The switchPlot",
        "Genome-wide Summaries",
        "Consequence Enrichment Analysis",
        "Splicing Enrichment Analysis",
        "Overview Plots",
        "Detailed Workflow",
        "Overview of Detailed Workflow",
        "IsoformSwitchAnalyzeR Background Information",
        "The switchAnalyzeRlist",
        "Function overview",
        "Importing Data Into R",
        "Importing Data from Kallisto, Salmon, RSEM or StringTie",
        "Importing Data from Salmon via Tximeta",
        "Importing Data from Cufflinks/Cuffdiff",
        "Importing Data from Full-length Transcript Assemblers",
        "Filtering",
        "Identifying Isoform Switches",
        "Testing for Isoform Switches via DEXSeq",
        "Testing for Isoform Switches via satuRn",
        "Testing for Isoform Switches with other Tools",
        "Analyzing Open Reading Frames",
        "Analyzing Only Known Isoforms",
        "Analyzing Known and Novel Isoforms",
        "Analyzing Only Novel Isoforms",
        "Extracting Nucleotide and Amino Acid Sequences",
        "Advice for Running External Sequence Analysis Tools and Downloading Results",
        "Importing External Sequence Analysis",
        "Predicting Alternative Splicing",
        "Predicting Switch Consequences",
        "Post Analysis of Isoform Switches with Consequences",
        "Analysis of Individual Isoform Switching",
        "Genome-Wide Analysis of Isoform Switching",
        "Other Tools in IsoformSwitchAnalyzeR",
        "Analyzing Alternative Splicing",
        "Overview of Alternative Splicing Workflow",
        "Genome-Wide Analysis of Alternative Splicing",
        "Interoperability with Other Tools",
        "pairedGSEA",
        "Importing results from pairedGSEA",
        "Exporting data to pairedGSEA",
        "Other workflows",
        "Applying on Long-read Sequencing Data",
        "Applying on Single-cell Sequencing Data",
        "Augmenting ORF Predictions with Pfam Results",
        "Analyze Small Upstream ORFs",
        "Remove Sequences Stored in SwitchAnalyzeRlist",
        "Adding Uncertain Category to Coding Potential Predictions",
        "Quality control of ORF of known annotation",
        "Analyzing the Biological Mechanisms Behind Isoform Switching",
        "Analyzing experiments without replicates",
        "Rescue StringTie Annoation and Extract Gene Count Matrix",
        "Frequently Asked Questions, Problems and Errors",
        "FAQ Table of Content",
        "What Quantification Tool(s) Should I Use?",
        "What Transcript Database Should I Use?",
        "Are there Different Approaches to Analysis of Alternative Splicing?",
        "Approach 1: Exon based analysis",
        "Approach 2: Splicing based analysis",
        "Approach 3: Transcript based analysis",
        "How to handle confounding effects (including batches)",
        "What constitute an independent biological replicate?",
        "Adding differential gene expression",
        "The error \"The annotation and quantification (count/abundance matrix and isoform annotation) seems to be different (Jaccard similarity < 0.925)\"",
        "The warning \"The annotation and quantification (count/abundance matrix and isoform annotation) seem to be slightly different\"",
        "The error \"The supplied design matrix will result in a model matrix that is not full rank\"",
        "How to prevent visualization of NMD in the switchPlot",
        "BSGenomes for non-model organisms",
        "The switchPlot contains regions \"Not Annotated\"",
        "Can I use a GFF file instead of a GTF file?",
        "Can I use quantificaiton files from Galaxy?",
        "Final Remarks",
        "Sessioninfo",
        "Generating additional links for sharing online"
      ],
      "created": "2017-04-18 15:35:39",
      "modified": "2025-12-01 10:36:14",
      "commits": 43
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