Package: maEndToEnd Title: An end to end workflow for differential gene expression using Affymetrix microarrays Version: 2.32.0 Date: 2024-01-12 Authors@R: c(person(role="aut", "Bernd", "Klaus", email = "bernd.klaus@embl.de"), person(role=c("aut", "cre"), "Stefanie", "Reisenauer", email = "steffi.reisenauer@tum.de")) Description: In this article, we walk through an end-to-end Affymetrix microarray differential expression workflow using Bioconductor packages. This workflow is directly applicable to current "Gene" type arrays, e.g. the HuGene or MoGene arrays, but can easily be adapted to similar platforms. The data analyzed here is a typical clinical microarray data set that compares inflamed and non-inflamed colon tissue in two disease subtypes. For each disease, the differential gene expression between inflamed- and non-inflamed colon tissue was analyzed. We will start from the raw data CEL files, show how to import them into a Bioconductor ExpressionSet, perform quality control and normalization and finally differential gene expression (DE) analysis, followed by some enrichment analysis. Depends: R (>= 3.5.0), Biobase, oligoClasses, ArrayExpress, pd.hugene.1.0.st.v1, hugene10sttranscriptcluster.db, oligo, arrayQualityMetrics, limma, topGO, ReactomePA, clusterProfiler, gplots, ggplot2, geneplotter, pheatmap, RColorBrewer, dplyr, tidyr, stringr, matrixStats, genefilter, openxlsx, Rgraphviz, enrichplot Suggests: BiocStyle, knitr, devtools, rmarkdown License: MIT + file LICENSE Encoding: UTF-8 VignetteBuilder: knitr biocViews: GeneExpressionWorkflow NeedsCompilation: no URL: https://www.bioconductor.org/help/workflows/ Workflow: True Config/pak/sysreqs: libcairo2-dev cmake libfontconfig1-dev libfreetype6-dev libfribidi-dev libglpk-dev make libharfbuzz-dev libicu-dev libjpeg-dev libpng-dev libuv1-dev libxml2-dev libssl-dev zlib1g-dev Repository: Bioconductor 3.23 Date/Publication: 2026-04-28 12:43:24 UTC RemoteUrl: https://github.com/bioc/maEndToEnd RemoteRef: RELEASE_3_23 RemoteSha: 4b49cad5e8cf18e1af79fdc06816b87ca8c0706f Packaged: 2026-10-07 14:48:13 UTC; root Author: Bernd Klaus [aut], Stefanie Reisenauer [aut, cre] Maintainer: Stefanie Reisenauer