Package: recountWorkflow Title: recount workflow: accessing over 70,000 human RNA-seq samples with Bioconductor Version: 1.36.0 Date: 2024-05-21 Authors@R: c(person("Leonardo", "Collado-Torres", role = c("aut", "cre"), email = "lcolladotor@gmail.com"), person("Abhinav", "Nellore", role = "ctb", email = "anellore@gmail.com"), person(c("Andrew", "E."), "Jaffe", role = "ctb", email = "andrew.jaffe@libd.org")) Description: The recount2 resource is composed of over 70,000 uniformly processed human RNA-seq samples spanning TCGA and SRA, including GTEx. The processed data can be accessed via the recount2 website and the recount Bioconductor package. This workflow explains in detail how to use the recount package and how to integrate it with other Bioconductor packages for several analyses that can be carried out with the recount2 resource. In particular, we describe how the coverage count matrices were computed in recount2 as well as different ways of obtaining public metadata, which can facilitate downstream analyses. Step-by-step directions show how to do a gene level differential expression analysis, visualize base-level genome coverage data, and perform an analyses at multiple feature levels. This workflow thus provides further information to understand the data in recount2 and a compendium of R code to use the data. Depends: R (>= 3.6.0) License: Artistic-2.0 Encoding: UTF-8 biocViews: Workflow, ResourceQueryingWorkflow VignetteBuilder: knitr Imports: recount, GenomicRanges, limma, edgeR, DESeq2, pheatmap, regionReport, clusterProfiler, org.Hs.eg.db, gplots, derfinder, GenomicState, bumphunter, derfinderPlot Suggests: BiocStyle, BiocWorkflowTools, knitr, magick, sessioninfo, rmarkdown NeedsCompilation: no Workflow: true URL: https://github.com/LieberInstitute/recountWorkflow BugReports: https://support.bioconductor.org/t/recountWorkflow/ RoxygenNote: 7.2.3 Config/pak/sysreqs: libcairo2-dev cmake libfontconfig1-dev libfreetype6-dev libglpk-dev make libbz2-dev libicu-dev liblzma-dev libpng-dev libuv1-dev libxml2-dev libssl-dev libx11-dev xz-utils zlib1g-dev Repository: Bioconductor 3.23 Date/Publication: 2026-04-28 12:43:06 UTC RemoteUrl: https://github.com/bioc/recountWorkflow RemoteRef: RELEASE_3_23 RemoteSha: 6ce0aaf55da837ec84d44daa78b0c92a6e5cf999 Packaged: 2026-10-04 17:14:38 UTC; root Author: Leonardo Collado-Torres [aut, cre], Abhinav Nellore [ctb], Andrew E. Jaffe [ctb] Maintainer: Leonardo Collado-Torres