Package: rnaseqGene Title: RNA-seq workflow: gene-level exploratory analysis and differential expression Version: 1.36.0 Date: 2026-04-13 Authors@R: c(person(role=c("aut", "cre"), "Michael", "Love", email = "michaelisaiahlove@gmail.com")) Description: Here we walk through an end-to-end gene-level RNA-seq differential expression workflow using Bioconductor packages. We will start from the FASTQ files, show how these were aligned to the reference genome, and prepare a count matrix which tallies the number of RNA-seq reads/fragments within each gene for each sample. We will perform exploratory data analysis (EDA) for quality assessment and to explore the relationship between samples, perform differential gene expression analysis, and visually explore the results. Depends: R (>= 3.3.0), BiocStyle, airway (>= 1.5.3), tximeta, magrittr, DESeq2, apeglm, vsn, dplyr, ggplot2, hexbin, pheatmap, RColorBrewer, PoiClaClu, glmpca, ggbeeswarm, genefilter, AnnotationDbi, org.Hs.eg.db, Gviz, sva, RUVSeq, fission License: Artistic-2.0 Encoding: UTF-8 VignetteBuilder: knitr biocViews: Workflow, GeneExpressionWorkflow, ImmunoOncologyWorkflow Suggests: knitr, rmarkdown NeedsCompilation: no URL: https://github.com/thelovelab/rnaseqGene/ Config/pak/sysreqs: cmake make libbz2-dev libicu-dev libjpeg-dev liblzma-dev libpng-dev libuv1-dev libxml2-dev libssl-dev xz-utils zlib1g-dev Repository: Bioconductor 3.23 Date/Publication: 2026-04-28 12:43:10 UTC RemoteUrl: https://github.com/bioc/rnaseqGene RemoteRef: RELEASE_3_23 RemoteSha: 19d2ab9e6a801bb8a7f22b250cf69d9023ac965b Packaged: 2026-10-04 17:14:30 UTC; root Author: Michael Love [aut, cre] Maintainer: Michael Love