# ------------------------------------------------ # CITATION.cff file created with {cffr} R package # See also: https://docs.ropensci.org/cffr/ # ------------------------------------------------ cff-version: 1.2.0 message: 'To cite package "seqpac" in publications use:' type: software license: GPL-3.0-only title: 'seqpac: Seqpac: A Framework for smallRNA analysis in R using Sequence-Based Counts' version: 1.12.0 doi: 10.1101/2021.03.19.436151 abstract: 'Seqpac provides functions and workflows for analysis of short sequenced reads. It was originally developed for small RNA analysis, but can be implemented on any sequencing raw data (provided as a fastq-file), where the unit of measurement is counts of unique sequences. The core of the seqpac workflow is the generation and subsequence analysis/visualization of a standardized object called PAC. Using an innovative targeting system, Seqpac process, analyze and visualize sample or sequence group differences using the PAC object. A PAC object in its most basic form is a list containing three types of data frames. - Phenotype table (P): Sample names (rows) with associated metadata (columns) e.g. treatment. - Annotation table (A): Unique sequences (rows) with annotation (columns), eg. reference alignments. - Counts table (C): Counts of unique sequences (rows) for each sample (columns). The PAC-object follows the rule: - Row names in P must be identical with column names in C. - Row names in A must be identical with row names in C. Thus P and A describes the columns and rows in C, respectively. The targeting system, will either target specific samples in P (pheno_target) or sequences in A (anno_target) and group them according to a target column in P and A, respectively (see vignettes for more details).' authors: - family-names: Isacson given-names: Signe email: signe.skog@liu.se - family-names: Natt given-names: Daniel email: daniel.natt@liu.se - family-names: Örkenby given-names: Lovisa email: lovisa.orkenby@liu.se - family-names: Gozzo given-names: Alessandro email: alessandro.gozzo@liu.se orcid: https://orcid.org/0000-0002-6466-627X - family-names: Asratian given-names: Anna email: anna.asratian@liu.se - family-names: Öst given-names: Anita email: anita.ost@liu.se preferred-citation: type: article title: 'Seqpac: A New Framework for small RNA analysis in R using Sequence-Based Counts' authors: - family-names: Skog given-names: Signe - family-names: Orkenby given-names: Lovisa - family-names: Kugelberg given-names: Unn - family-names: Tariq given-names: Kanwal - family-names: Farrants given-names: Ann-Kristin Ostlund - family-names: Ost given-names: Anita - family-names: Natt given-names: Daniel email: daniel.natt@liu.se journal: bioRxiv year: '2021' doi: 10.1101/2021.03.19.436151 volume: _ issue: _ start: _ repository: https://bioconductor.org/ repository-code: https://github.com/OestLab/seqpac commit: 32327358db6b9bfba00447e145fd26309278e7ba url: https://github.com/Oestlab/seqpac date-released: '2026-04-28' contact: - family-names: Gozzo given-names: Alessandro email: alessandro.gozzo@liu.se orcid: https://orcid.org/0000-0002-6466-627X